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KMID : 0364819880260030162
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Korean Journal of Microbiology
1988 Volume.26 No. 3 p.162 ~ p.166
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Inhibiton of SV40 DNA Replication in Simian Cell by Bacteriophage M13 DNA sequences
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Kim, Yeon-Soo/김연수
Koo, Yong-Eui/Kang, Hyen-Sam/구용의/강현삼
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Abstract
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1
Bacteriophage M13 DNAs carrying the wild type or base substituted SV4O DNA replication origins were used for replication assay. In vivo and in vitro assay with African green monkey cell line COS-1 showed that the replication of M13-SV40 recombinant DNAs was restricted like a pBR322SV4O recombinant DNA(Lusky and Botchan, 1981). Furthermore, recombinant phage DNAs isolated from the transtected shaman cells subsequently show a reduced ability to retransform ￡ coiL But pATSV-W(Kim t aL, 1918) was replicated in COS-1 cells normally. We think that a poison sequence may exist on bacteriophage M13 DNA like pBR322.
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